Question

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List the three types of artificial genetic manipulation. Which uses bacterial enzymes to cut DNA molecules in particular locations?

Answer 1

the three types of artificial genetic manipulation which uses bacterial enzymes to cut DNA molecules in particular locations are as follows:-

1) Insertion of restriction sites within the DNA molecules. Presence of non methylated restriction site determine the site where DNA molecule would get cut by restriction enzyme.

2) Mutation. Inserting mutation in one strand of DNA molecule lead to formation of  a “bubble” at the mismatch of the two DNA strands, which is then cleaved by a single stranded nucleases.

3) CRISPR/Cas system. This is newly develop technique for gene editing. CRISPR is a family of DNA sequences in bacteria. The sequences contain snippets of DNA from viruses that have attacked the bacterium and these snippets are these snippets are used by the bacterium to detect and destroy DNA from similar viruses during subsequent attacks through its CAS Protein.