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In regards to the Polymerase Chain Reaction: what is melting, annealing, extension

In regards to the Polymerase Chain Reaction: what is melting, annealing, extension

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Answer #1

Polymerase chain reaction is a reaction which is used to make copies of DNA.

There are various steps of PCR-

1. MELTING- The process in which two strands of DNA are separated by denaturing at high temperature. This process provides single-stranded DNA for the next process.

2. Annealing- the primers bind to their complementary sequences on the single-stranded template DNA and thus starts the polymerization

3. Extension- In this process primers start synthesizing the new complementary strand of DNA.

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