Question

In the polymerase chain reaction, DNA is heated to 95 degrees Celsius and nucleotides, enzymes and DNA primers are added to the mixture. What happens when DNA is heated to 95 degrees Celsius?

Answer 1

In a PCR reaction when a DNA is heated to 95 degree celcius the double stranded DNA unwinds, the heats breaks the hydrogen bonds between the complementry nucleotides of the two DNA strands and two strands of single stranded DNA are available this is the denaturation step in a polymerase chain reaction.

the next step is anneling step in which temprature of the reaction is lower enabeling attachment of DNA primers at specific location on the single stranded DNA starting the DNA synthesis process

in the extension step Dna polymerase adds nucleotides and starts making a complimentry strand to the existing template DNA strand.One cycle of PCR yields two double-stranded DNA, each containing one newly made strand and one original strand.