Question

what are the parameters involved in Selecting an annealing temperature based on the Tm of forward...

what are the parameters involved in Selecting an annealing temperature based on the Tm of forward and reverse primers and selecting an extension time based on the expected length of PCR products, justify your selection

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Ans 1-Parameters involved in selecting annealing temperature based on Tm of forward and reverse primers are - Lenght and composition of primers . Longer strands have higher melting temperatures, as do sequences with higher G and C content.

Ans 2- Parameters involved in selecting extension time based on the expected Lenght of PCR products are as follows- number of base pairs. Use extension time of 1 minute per thousand base pairs eg. 3 minutes for a 3 kb product. For products less than 1 kb , use 45-60 seconds. Products greater than 3 kb or reactions using more than 30 cycles , may require longer extensions.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
You are performing a simple polymerase chain reaction (PCR) to amplify ANY 300 - 500 bp...
You are performing a simple polymerase chain reaction (PCR) to amplify ANY 300 - 500 bp region (for example nucleotide 5 to 555 or 20 to 575). Using your primers, you will run a theoretical PCR reaction, and then run the product on an agarose gel to determine if your PCR reaction worked correctly. CAGGAAACAGCTATGACCATGATTACGCCAAGCTTGGTACCGAGCTCGGATCCACTAGTA ACGGCCGCCAGTGTGCTGGAATTCGGCTTCCCGGAGCCGGACCGGGGCCACCGCGCCCGC TCTGCTCCGACACCGCGCCCCCTGGACAGCCGCCCTCTCCTCCAGGCCCGTGGGGCTGGC CCTGCACCGCCGAGCTTCCCGGGATGAGGGCCCCCGGTGTGGTCACCCGGCGCGCCCCAG GTCGCTGAGGGACCCCGGCCAGGCGCGGAGATGGGGGTGCACGAATGTCCTGCCTGGCTG TGGCTTCTCCTGTCCCTGCTGTCGCTCCCTCTGGGCCTCCCAGTCCTGGGCGCCCCACCA CGCCTCATCTGTGACAGCCGAGTCCTGGAGAGGTACCTCTTGGAGGCCAAGGAGGCCGAG AATATCACGACGGGCTGTGCTGAACACTGCAGCTTGAATGAGAATATCACTGTCCCAGAC ACCAAAGTTAATTTCTATGCCTGGAAGAGGATGGAGGTCGGGCAGCAGGCCGTAGAAGTC TGGCAGGGCCTGGCCCTGCTGTCGGAAGCTGTCCTGCGGGGCCAGGCCCTGTTGGTCAAC TCTTCCCAGCCGTGGGAGCCCCTGCAGCTGCATGTGGATAAAGCCGTCAGTGGCCTTCGC AGCCTCACCACTCTGCTTCGGGCTCTGCGAGCCCAGAAGGAAGCCATCTCCCCTCCAGAT CAGCCTGTCCCATGGACACTCCAGTGCCAGCAATGACATCTCAGGGGCCAGAGGAACTGT CCAGAGAGCAACTCTGAGATCTAAGGATGTCACAGGGCCAACTTGAGGGCCCAGAGCAGG AAGCATTCAGAGAGCAGCTTTAAACTCAGGGACAGAGCCATGCTGGGAAGACGCCTGAGC TCACTCGGCACCCTGCAAAATTTGATGCCAGGACACGCTTTGGAGGCGATTTACCTGTTT TCGCACCTACCATCAGGGACAGGATGACCTGGAGAACTTAGGTGGCAAGCTGTGACTTCT CCAGGTCTCACGGGCATGGGCACTCCCTTGGTGGCAAGAGCCCCCTTGACACCGGGGTGG TGGGAACCATGAAGACAGGATGGGGGCTGGCCTCTGGCTCTCATGGGGTCCAAGTTTTGT GTATTCTTCAACCTCATTGACAAGAACTGAAACCACCAAAAAAAAAAAAAAAAGCCGAAT TCTGCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCAATTCGC CCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAA TCTTCCCAGCCGTGGGAGCCCCTGCAGCTGCATGTGGATAAAGCCGTCAGTGGCCTTCGC AGCCTCACCACTCTGCTTCGGGCTCTGCGAGCCCAGAAGGAAGCCATCTCCCCTCCAGAT GCGGCCTCAGC Questions...
PCR Questions What gives DNA strands its polarity? To answer, tell me what the “ 5’...
PCR Questions What gives DNA strands its polarity? To answer, tell me what the “ 5’ ” and “ 3’ ” ends are referring to in chemical terms.   Diagram a “template” and the primers for a PCR reaction, including the 5’ and 3’ notations as appropriate. Will the “forward” primer have the same sequence as the coding strand or complement strand? Will the “reverse” primer have the same sequence as the coding strand or complement strand? After running PCR, a...
What is your opinion on this statement based on the idea of what audit steps would...
What is your opinion on this statement based on the idea of what audit steps would you perform to evaluate the contract bidding process? While auditing an organizations contract bidding process, I would make a preliminary survey, plan the audit approach, and establish audit objectives. In my preliminary survey, I would interview key personnel who are involved in the procurement process, such as the chief procurement officer or similar staff, review the purchasing policy of the organization, and determine what...
1). What were the justifications given for funding the Human Genome Project? A). Scientific discoveries B)....
1). What were the justifications given for funding the Human Genome Project? A). Scientific discoveries B). Facilitate technological advances C). Understanding and treating human diseases D). Economic impact E). All of the above. 2). What are some of the concerns with using transcriptional Fusion reporter gene constructs to following the expression of the gene of Interest? A ). The reporter gene fused to the gene of Interest may interfere with the proper folding and function of protein product of the...
After reading the following article, how would you summarize it? What conclusions can be made about...
After reading the following article, how would you summarize it? What conclusions can be made about Amazon? Case 12: Amazon.com Inc.: Retailing Giant to High-Tech Player? (Internet Companies) Overview Founded by Jeff Bezos, online giant Amazon.com, Inc. (Amazon), was incorporated in the state of Washington in July 1994, and sold its first book in July 1995. In May 1997, Amazon (AMZN) completed its initial public offering and its common stock was listed on the NASDAQ Global Select Market. Amazon quickly...
What strategy would you recommend for Dove (1) in responding (or in not responding) to consumers...
What strategy would you recommend for Dove (1) in responding (or in not responding) to consumers and (2) going forward (or not going forward) with the campaign? If they were to go forward with this campaign, should Dove revise its Real Beauty message and if, so, how? The Dove Real Beauty Campaign In 2004, with sales at approximately $2.5 billion, Dove saw an opportunity to tap into women’s self-perceptions of beauty to address female insecurities while building a connection between...
What are 4 key things you learned about the topic from reading their paper? How does...
What are 4 key things you learned about the topic from reading their paper? How does the topic relate to you and your current or past job? Critique the paper in terms of the organization and quality. Team 3 answer questions above. Part I In today’s world we see fear among people when dealing with sexual harassment. This leads to people not reporting sexual harassment. A misconception about sexual harassment is that it’s only about touching and forcing other people...
The questions: 1. What type of technology Acme and Omega utilize to transform inputs into outputs?...
The questions: 1. What type of technology Acme and Omega utilize to transform inputs into outputs? 2. Which strategic choice (differentiation or cost leadership) suits best to Acme? Omega? Do these companies have clear strategic choices or do they stuck in the middle? 3. Based on all the contingencies which type of structure is more suitable for these companies; mechanistic or organic? please answer each question alone The Paradoxical Twins: Acme and Omega Electronics John F. Veiga Part! boom of...
Which of the following best describes a project management plan? The project charter, WBS, and project...
Which of the following best describes a project management plan? The project charter, WBS, and project scope statement The schedule, management plans, and budget A formal, approved document used to control the project The project manager's plan for managing and controlling the work The engineering department wants the project objective to be a 10 percent improvement in throughput. The information technology department wants no more than 5 percent of its resources to be used on the project. Management, who is...
1. The failure of the new supply chain system affected Nike adversely. What were the reasons...
1. The failure of the new supply chain system affected Nike adversely. What were the reasons for the failure and how did the breakdown harm Nike? 2. What are the important elements to be kept in mind while implementing a new system in an organization? What is the importance of a good working relationship between partners and the sharing of responsibility in implementing critical projects? What mistakes did Nike and i2 make? 3. comment on the lessons learned and the...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT