Question

Discuss how the understanding of DNA replication in organisms has been applied in Polymerase Chain Reaction (PCR) techniques. How is PCR different from what happens during in vivo DNA replication?

Answer 1

Differences between PCR and in Vivo DNA replication are as follows

a. Different in the Machinery

DNA replication takes place at 37 degree in human and has the complex machinery. But in PCR reaction temperature of 70-90C) is used for the denaturation and annealing of DNA strands.

b. Difference in polymerase - There a various types DNA polymerases that is used on replication in Vivo but in PCR single DNA polymerases is used that is called as Taq polymerase

c. Differencecin Length of DNA -

In the in vivo process thr Whole genomic DNA is replicated but PCR only smaller fragments of DNA are amplified