Question

The goal of the polymerase chain reaction (PCR) is to:

		Cleave DNA molecules
		Digest bacterial plasmids
		Amplify a small amount of DNA sequence
		Sequence DNA

Answer 1

PCR stands for Polymerase Chain Reaction. It is technique/method by which we carry out multiple successive cycles of DNA replication by using a heat-tolerant polymerase from a bacteria found in hot-springs called Thermus aquaticus. Even a small amount of DNA will suffice and with every cycle, the initial DNA material doubles in number. As such it follows the same growth kinetics as that of bacteria and their doubling time. Thus we manage to achieve a amplification of a large magnitude with very dew cycles. Example 10 cycles gives us 1024-fold amplification, 30 cycles gives us 1,073,741,824-fold amplification, that is 'n' cycles gives us 2ⁿ-fold amplification?.
The goal of the polymerase chain reaction (PCR) is to:
Amplify a small amount of DNA sequence ANSWER