Question

what are Polymerase chain reaction requirements, (template DNA, dNTPs, primers)

Answer 1

1) The DNA template is that particular DNA sequence which you want to be amplified.

2) DNA polymerase enzyme for PCR that has a good activity rate around 75°C and it should be able to withstand temperatures of 95-100°C.

3) Primers - they are short oligonucleotides of DNA, usually around 8-40 base pairs in length. the purpose of this is to amplify a certain section of DNA.

4) four different deoxyribonucleotide triphosphates (dNTPs), adenine (A), guanine (G), cytosine (C), and thymine (T) are needed to provide the raw material for synthesis.

5) a reaction buffer is used to provide a stable pH.

6) a divalent cation usually Mg⁺² plays a vital role in the PCR reaction, acting as a co-factor for DNA polymerase enzyme.