Question

What occurs during the annealing stage of a PCR reaction? A. The reaction stops B. The...

  1. What occurs during the annealing stage of a PCR reaction?

    A.

    The reaction stops

    B.

    The DNA polymerase copies the template DNA

    C.

    The primers bind to the template DNA.

    D.

    The DNA strands separate

  2. The PCR protocol that is currently used in laboratory was facilitated by a discovery of bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park, in Wyoming. This organism contains a heat stable form of DNA Polymerase known as Taq polymerase, which continues to function even after it has been heated to 95oC. Why would such a heat stable polymerase be beneficial in PCR?
  3. A.

    Each cycle includes a hot saturation phase (95 ◦C), which allows the primers to anneal to the target DNA

    B.

    Each cycle includes a hot denaturation phase (95C), which separates the hydrogen bonds that hold the strands of template DNA together

    C.

    Each cycle includes a hot denaturation phase (95 ◦C), which activates the Taq polymerase

    D.

    Each cycle includes a hot denaturation phase (95 ◦C), which serves to sterilize the culture

Homework Answers

Answer #1

1. What occurs during the annealing stage of a PCR reaction?

The primers bind to the template DNA.

During annealing stage of PCR (after denaturation of DNA), primers bind to the cDNA (DNA template).

2. Why would such a heat stable polymerase be beneficial in PCR?

Each cycle includes a hot denaturation phase (95°C), which separates the hydrogen bonds that hold the strands of template DNA together.

Heating at 95°C breaks down hydrogen bonds between nitrogenous bases of dsDNA to generate ssDNA (DNA template).

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Match the following terms or phrases with the corresponding stage of PCR: Denaturation; Annealing; extension 1.Taq...
Match the following terms or phrases with the corresponding stage of PCR: Denaturation; Annealing; extension 1.Taq polymerase is adding nuclueotides 2.Primers bind to flanking sequences 3.Approximately 95 degrees Celcius 4.Approximately 60 degrees Celsius 5.Approximately 75 degrees Celcius 6.Hydrogen bonds are broken between double-stranded DNA
Which are components of a PCR reaction? Check All That Apply RNA primers Dideoxyribonucleotides DNA ligase...
Which are components of a PCR reaction? Check All That Apply RNA primers Dideoxyribonucleotides DNA ligase Heat–stable DNA polymerase Template DNA Deoxyribonucleotides
PCR is a technique to amplify targeted segments of DNA in vitro. The denaturation step during...
PCR is a technique to amplify targeted segments of DNA in vitro. The denaturation step during the cycle does the job of which of the enzymes below? A) Ligase B) Helicase C) Primase D) Taq polymerase
PCR Questions What gives DNA strands its polarity? To answer, tell me what the “ 5’...
PCR Questions What gives DNA strands its polarity? To answer, tell me what the “ 5’ ” and “ 3’ ” ends are referring to in chemical terms.   Diagram a “template” and the primers for a PCR reaction, including the 5’ and 3’ notations as appropriate. Will the “forward” primer have the same sequence as the coding strand or complement strand? Will the “reverse” primer have the same sequence as the coding strand or complement strand? After running PCR, a...
You are performing a simple polymerase chain reaction (PCR) to amplify ANY 300 - 500 bp...
You are performing a simple polymerase chain reaction (PCR) to amplify ANY 300 - 500 bp region (for example nucleotide 5 to 555 or 20 to 575). Using your primers, you will run a theoretical PCR reaction, and then run the product on an agarose gel to determine if your PCR reaction worked correctly. CAGGAAACAGCTATGACCATGATTACGCCAAGCTTGGTACCGAGCTCGGATCCACTAGTA ACGGCCGCCAGTGTGCTGGAATTCGGCTTCCCGGAGCCGGACCGGGGCCACCGCGCCCGC TCTGCTCCGACACCGCGCCCCCTGGACAGCCGCCCTCTCCTCCAGGCCCGTGGGGCTGGC CCTGCACCGCCGAGCTTCCCGGGATGAGGGCCCCCGGTGTGGTCACCCGGCGCGCCCCAG GTCGCTGAGGGACCCCGGCCAGGCGCGGAGATGGGGGTGCACGAATGTCCTGCCTGGCTG TGGCTTCTCCTGTCCCTGCTGTCGCTCCCTCTGGGCCTCCCAGTCCTGGGCGCCCCACCA CGCCTCATCTGTGACAGCCGAGTCCTGGAGAGGTACCTCTTGGAGGCCAAGGAGGCCGAG AATATCACGACGGGCTGTGCTGAACACTGCAGCTTGAATGAGAATATCACTGTCCCAGAC ACCAAAGTTAATTTCTATGCCTGGAAGAGGATGGAGGTCGGGCAGCAGGCCGTAGAAGTC TGGCAGGGCCTGGCCCTGCTGTCGGAAGCTGTCCTGCGGGGCCAGGCCCTGTTGGTCAAC TCTTCCCAGCCGTGGGAGCCCCTGCAGCTGCATGTGGATAAAGCCGTCAGTGGCCTTCGC AGCCTCACCACTCTGCTTCGGGCTCTGCGAGCCCAGAAGGAAGCCATCTCCCCTCCAGAT CAGCCTGTCCCATGGACACTCCAGTGCCAGCAATGACATCTCAGGGGCCAGAGGAACTGT CCAGAGAGCAACTCTGAGATCTAAGGATGTCACAGGGCCAACTTGAGGGCCCAGAGCAGG AAGCATTCAGAGAGCAGCTTTAAACTCAGGGACAGAGCCATGCTGGGAAGACGCCTGAGC TCACTCGGCACCCTGCAAAATTTGATGCCAGGACACGCTTTGGAGGCGATTTACCTGTTT TCGCACCTACCATCAGGGACAGGATGACCTGGAGAACTTAGGTGGCAAGCTGTGACTTCT CCAGGTCTCACGGGCATGGGCACTCCCTTGGTGGCAAGAGCCCCCTTGACACCGGGGTGG TGGGAACCATGAAGACAGGATGGGGGCTGGCCTCTGGCTCTCATGGGGTCCAAGTTTTGT GTATTCTTCAACCTCATTGACAAGAACTGAAACCACCAAAAAAAAAAAAAAAAGCCGAAT TCTGCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCAATTCGC CCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAA TCTTCCCAGCCGTGGGAGCCCCTGCAGCTGCATGTGGATAAAGCCGTCAGTGGCCTTCGC AGCCTCACCACTCTGCTTCGGGCTCTGCGAGCCCAGAAGGAAGCCATCTCCCCTCCAGAT GCGGCCTCAGC Questions...
Procedure I. Reverse Transcription. For each of your RNA samples set up the following reaction: *Remember...
Procedure I. Reverse Transcription. For each of your RNA samples set up the following reaction: *Remember to use proper RNA handling technique! 1. In a 0.2 ml size RNAse-Free tube add the following: • 0.5 µg RNA (no more than 6 µl) You can add as little as 0.1 µg of RNA if your concentration is low • 2 µl dT primer • Nuclease Free Water to final volume of 8 µl * Before you start write the exact volume...
1.What is the major component of blood plasma? a Nutrients b Ions c Water d Gases...
1.What is the major component of blood plasma? a Nutrients b Ions c Water d Gases 2. How many layers of phospholipids does the cell membrane contain? a 1 b 2 c 4 d 3 3. Which substance stores polysaccharides in plants? a Sucrose b Glycogen c Glucose d Cellulose 4. An important concept in DNA and RNA structure is complementary base pairing. Which tow bases are incorrectly paired? a T-U b A-T c C-G d A-U 5. If the...