Question

what is the conclusion of Staphylococcus saprophyticus on a DNA agar test? (positive or Negative)

Answer 1

ANSWER ) Negative

EXPLANATION

DNase Test Agar is used to differentiate
between microorganisms based on their ability to
produce the enzyme deoxyribonuclease (DNase). DNase agar is a differential medium that tests the ability of an organism to produce an exoenzyme, called deoxyribonuclease or DNase, that hydrolyzes DNA. DNase agar contains nutrients for the bacteria, DNA, and methyl green as an indicator. Methyl green is a cation which binds to the negatively-charged DNA.When the DNA is broken down, it no longer binds to the methyl green, and a clear halo will appear around the areas where the DNase-producing organism has grown.

When Staphylococcus saprophyticus grown on a DNA agar there is no breakdown of the DNA in the agar. After adding the HCl, the entire plate turned cloudy as the DNA precipitated out of solution. There is no clear zone around the bacterial growth.