Question

In which direction does the DNA run on the gel? Toward the positive or negative pole?...

In which direction does the DNA run on the gel? Toward the positive or negative pole? Why?

2. If you plug the read lead into the (-) source and the black lead into the (+) source on the power supply, what will happen to your DNA when you run your gel?

.3. What is the purpose of the loading dye?

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Answer #1

1) DNA are negatively charged molecules due to the presence of phosphate groups in their sugar-phosphate backbone, so they start moving through the matrix of the gel towards the positive pole.

2) The DNA will move out of the gel and no bands will be visible.

3) Loading dye is mixed with DNA samples for visual tracking of DNA migration during electrophoresis. The loading dye contains two different dyes for tracking of the DNA, glycerol to ensure that the DNA in the ladder and sample forms a layer at the bottom of the well and EDTA binds divalent metal ions and inhibits metal-dependent nucleases.

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