Question

TEST	Result or NA (if not applicable)	Observation (Color/Appearance/full description)
STREAK PLATE (Nutrient Agar)	Smooth, convex, mucoid round colonies with red pigment
Simple Stain (to check arrangement)	Bacilli	Rod shape
TSA (Tryptic Soy Agar)	Smooth, round colonies with red pigmentation
Sabaroud Dextrose Agar (SDA)	N/A	Medium for fungi
GRAM STAIN	Gram-negative rod	Pink color after staining
Thioglycollate Broth	Facultative anaerobe	Grow distributed throughout the broth Broth show turloid growth
PEA (Phenylethyl Alcohol Agar)	No growth	Gram-negative inhibited by alcohol content
Mannitol Salt Agar (MSA)	No growth	High salt content inhibits the growth
Blood Agar	Depends on strain Some are beta-hemolytic Mostly are gamma hemolytic	Colorless colonies on blood agar

TEST	Result or NA (if not applicable)	Observation (Color/Appearance/ full description)
BEA Slant (Bile Esculetin)	Positive	Esculin is hydrolyzed to esculetin and dextrose which react with FeCl2 forming black brown color
SIM (identify all 3 test results)	No H2S production Indole Negative Motile	No red color band No black precipitates formation Diffuse growth show motility
Catalase Test	Positive	The bubbling of gases show that it can decompose H2O2 H2O SO4
Phenyl Red Glucose or Sucrose Fermentation Tube (w/ Durham)	Acid gas Acid	Color change from red to yellow + gas bubbles Color change from red to yellow
Oxidase Test	Negative	It does not produce Cytochrome oxidase enzyme No purple color
Simon Citrate Test	Positive	It can use citrate as the sole source of carbon Prussian blue color change
Acid Fast Stain	N/A
Eosin Mythylene Blue (EMB)	Growth has seen, mostly non-lactose fermenting colonies
TOTAL POINTS FOR OTHER TESTS

Unknown bacteria: Serratia marcescens

*Need help drawing dichotomous key using the information above:

Answer 1