Question

Which of the following is NOT a mechanism employed by enzymes to lower the energy of the transition state?

High substrate binding affinity ?

General acid/base catalysis?

Metal ion catalysis ?

Covalent catalysis ?

Proximity and orientation effects?

Answer 1

1.High substrate binding affinity results to faster rate of enzymatic reactions. It increases the rate of reaction but not affect on transition state.

2.general acid base cataylsis and metal ion catalysis lowers the energy of transition State via interaction with transition state.

3. Covelent catalysis doesn't lowers the energy of transition state, but it provide an alternate route of reaction having lowers energy barrier.

4. Proximity and orientation increase the rate of the reaction by increasing the rate of effective collision between the substrate. This is also not involve in lowering of transition state energy.

Acid/base cataylsis and metal ion catalysis works by lowering the energy of transition state.