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What molecular biology principle is the basis for DNA fingerprinting?

What molecular biology principle is the basis for DNA fingerprinting?

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Answer #1

DNA fingerprinting (DNA typing or genotyping) is a method used to identify an individual from a sample of DNA by looking at unique patterns in theor DNA. These unique, individual patterns of DNA are the result of Mendelian inheritance of polymorphic, hypervariable loci of repetitive DNA.

The steps involved are:

1. Extract DNA from sample material

2. Restriction enzymes used to cut DNA

3. These small piece of DNA are run in agarose gel electrophoresis.

4.DNA pieces are blotted onto a nitrocellulose membrane

5. Membrane incubated with radioactive probes

6.Probes are visualised in a X-ray film.

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