Question

On what basis are proteins separated in electrophoresis?

  1. On what basis are proteins separated in electrophoresis?

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Protein electrophoresis is when proteins in a mixture are separated under the influence of an electric field. In the nondenaturing state (native gel) proteins are separated by their charge to mass ratio, shape, and size. While in the denatured state (SDS PAGE) proteins are unfolded by the detergent SDS (sodium dodecyl sulfate). Then SDS binds to the denatured polypeptides and evenly distribute the charges according to their mass. Therefore in SDS-PAGE, separation of protein is based on their size.

SDS PAGE figure--

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
In paper electrophoresis, you separated amino acids based on charge. What is the variable this time...
In paper electrophoresis, you separated amino acids based on charge. What is the variable this time in SDS-PAGE? How do you know that there is only one variable in this separation? Explain in detail.
1. Agarose gel electrophoresis can separate: A. DNA B. Protein C. DNA or proteins 2. Polyacrylamide...
1. Agarose gel electrophoresis can separate: A. DNA B. Protein C. DNA or proteins 2. Polyacrylamide gel electrophoresis can separate: A. DNA B. Protein C. DNA or proteins
On completion of the separation of proteins by polyacrylamide gel electrophoresis (PAGE), the next step is...
On completion of the separation of proteins by polyacrylamide gel electrophoresis (PAGE), the next step is to transfer the proteins from the gel to a solid support membrane, usually made of a chemically inert substance, such as : Select one: a. Nitrocellulose b. Filter paper c. β-Mercaptoethanol d. Acrylamide
Explain how treatment of proteins with sodium dodecyl sulphate (SDS) enables their separation by gel electrophoresis.
Explain how treatment of proteins with sodium dodecyl sulphate (SDS) enables their separation by gel electrophoresis.
Can you separate a mixture of the following three proteins using membrane electrophoresis? Protein A has...
Can you separate a mixture of the following three proteins using membrane electrophoresis? Protein A has a pI > pH of the run buffer, Protein B has a pI < the pH of the run buffer, and Protein C has a pI = pH of the run buffer. If so, describe the movement of each protein.
What is electrophoresis
What is electrophoresis
How is information encoded in DNA used to generate proteins? What is the molecular basis of...
How is information encoded in DNA used to generate proteins? What is the molecular basis of the genetic code?
In electrophoresis, what are the factors affecting electrophoretic resolution?
In electrophoresis, what are the factors affecting electrophoretic resolution?
What is the purpose of a molecular weight marker in gel electrophoresis
What is the purpose of a molecular weight marker in gel electrophoresis
What is the purpose of ethidium bromide in agarose gel electrophoresis?
What is the purpose of ethidium bromide in agarose gel electrophoresis?
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT