Question

What is the purpose of ethidium bromide in agarose gel electrophoresis?

What is the purpose of ethidium bromide in agarose gel electrophoresis?

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Answer #1

Purpose of ethidium bromide in agarose gel electrophoresis is that:
It is added to the running buffer solution during the separation of DNA fragments by agarose gel electrophoresis.
Ethidium bromide binds by intercalating between DNA base pairs,  thus acting as a visualising agent for DNA due to it's fluroscent property that it shows when illuminated with Ultra violet light , and the flluorscent images are recorded as photographs thereby making DNA bands visible. So, we say ethidium bromide acts as an visualising agent for DNA.

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