Question

1. Agarose gel electrophoresis can separate: A. DNA B. Protein C. DNA or proteins 2. Polyacrylamide...

1. Agarose gel electrophoresis can separate:

A. DNA

B. Protein

C. DNA or proteins

2. Polyacrylamide gel electrophoresis can separate:

A. DNA

B. Protein

C. DNA or proteins

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Answer #1
  1. The size of DNA is larger than proteins and hence DNA is separated by agarose gel electrophoresis as the porosity of agarose is large. So, the correct option is option A, DNA.
  2. Proteins are smaller molecules compared to DNA. Hence, they are separated by polyacrylamide gel electrophoresis as the porosity of acrylamide gel is small. Further, DNA molecules with a molecular weight of less than 500Kda can also be separated using polyacrylamide gel electrophoresis. So, the correct option is option C, DNA or proteins.
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