Question

On completion of the separation of proteins by polyacrylamide gel electrophoresis (PAGE), the next step is to transfer the proteins from the gel to a solid support membrane, usually made of a chemically inert substance, such as :

Select one:

a. Nitrocellulose

b. Filter paper

c. β-Mercaptoethanol

d. Acrylamide

Answer 1

PAGE = it is a kind of electrophoresis in which polyacrylamide gel is used which is prepared by addition of water with acrylamide powder. In it separation of proteins is done one the basis of their size.

After the protein separation is done, the next step is to transfer the proteins from the gel to a solid support membrane, which is chemically inert is ------- option A, Nitrocellulose.

This transfer to a solid support is an important step as : 1) provide better handling. As the separated Proteins are on gel so on transferring to a solid matrix helps their fixing and will be easy to handle.

2) it also provide better target accessibility for other macromolecules.

The solid matrix generally used are Nitrocellulose, nylon and PVDF ( polyvinyldiene difluoride).

So, correct answer Option A, Nitrocellulose