Question

In electrophoresis, what are the factors affecting electrophoretic resolution?

Answer 1

Some of the factors that affect the electrophoretic resolution includes Factors affecting electrophoresis include characteristics of the ion or molecule itself, the environment (buffer) in which the molecule or ions are being studied, and the applied electrical field. And to be noted that these factors specifically affect the migration rates of the molecules in the sample during electrophoresis.And also Electrophoretic mobility depends on the Charge: higher the charge greater the electrophoretic mobility. Size: bigger the molecule, greater are the frictional and electrostatic forces exerted on it by the medium.so these are the various factors affecting the electrophoretic resolution.