Question

1. What enzyme is used to make cDNA?

restriction enzymes

reverse transciptase

DNA polymerase

RNA polymerase

2. Which of the following would you NOT need to put in a test tube to do Polymerase Chain Reaction?

helicase

Taq polymerase

nucleotides

primers

3. What percentage of the human genome codes for proteins, tRNA and rRNA?

100%

1 %

0.1%

10%

4. In gene therapy what is used to get the good copy of the gene into tha patients cells?

a "gene gun"

surgery

a virus

a bacterial plasmid

4.Approximately how many genes are found in the human genome?

		21,000
		200,000
		2 million
		200

Answer 1

1. Reverse transcriptase

cDNA is synthesised from a single stranded mRNA or microRNA template in a reaction which is catalysed by the enzyme reverse transcriptase. It is often used to clone eukaryotic genes into prokaryotes.

2. Helicase

This is used to unwind the DNA helix during replication. So, it will hinder in the process of polymerisation.

3. About 1%

4. Gene gun

Earlier viruses like retrovirus were used to introduce new gene into a cell. With advance in technology gene gun has become a more used technology to introduce new genes into a cell.

5. About 21,000 to 30,000 genes