Question

During the PCR reaction, the DNA sample is heated in order to separate it into single...

During the PCR reaction, the DNA sample is heated in order to

separate it into single strands.

allow primers to bind.

synthesize RNA.

allow DNA polymerase to work.

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

During PCR, the DNA sample is heated in order to separate it into single strands.

This is the first step of PCR which is called as Denaturation. In this step, hydrogen bonds between strands are broken by applying external heat. Heating eradicates the need of helicase to separate DNA strands.

Whole Polymerase chain Reaction occurs in three steps.

1. Denaturation: Here DNA strand is heated(94-95?) to separate in to single strands.

2. Annealing: Here the temperature of reaction mixture is lowered( 50-65?)which allows binding of DNA primers to templete DNA.

3. Extension: Here the temperature is slightly raised( 72?)and synthesis of new strands takes place by the activity of Taq polymerase enzymes.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
What occurs during the annealing stage of a PCR reaction? A. The reaction stops B. The...
What occurs during the annealing stage of a PCR reaction? A. The reaction stops B. The DNA polymerase copies the template DNA C. The primers bind to the template DNA. D. The DNA strands separate The PCR protocol that is currently used in laboratory was facilitated by a discovery of bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park, in Wyoming. This organism contains a heat stable form of DNA Polymerase known as Taq polymerase, which continues...
Which are components of a PCR reaction? Check All That Apply RNA primers Dideoxyribonucleotides DNA ligase...
Which are components of a PCR reaction? Check All That Apply RNA primers Dideoxyribonucleotides DNA ligase Heat–stable DNA polymerase Template DNA Deoxyribonucleotides
In the polymerase chain reaction, DNA is heated to 95 degrees Celsius and nucleotides, enzymes and...
In the polymerase chain reaction, DNA is heated to 95 degrees Celsius and nucleotides, enzymes and DNA primers are added to the mixture. Why are DNA primers added during the polymerase chain reaction?
1.What is the role of telomerase in DNA replication? a) join Okazaki fragments together b) synthesize...
1.What is the role of telomerase in DNA replication? a) join Okazaki fragments together b) synthesize RNA nucleotides to make a primer c) catalyze the lengthening of the ends of linear chromosomes d) are part of the initiation of replication at the origin of replication 2. RNA polymerase for mRNA transcription and DNA polymerase in replication differ in that a) RNA polymerase uses RNA as a template, and DNA polymerase uses a DNA template. b) RNA polymerase does not need...
In the polymerase chain reaction, DNA is heated to 95 degrees Celsius and nucleotides, enzymes and...
In the polymerase chain reaction, DNA is heated to 95 degrees Celsius and nucleotides, enzymes and DNA primers are added to the mixture. What happens when DNA is heated to 95 degrees Celsius?
The difference between PCR and real-time PCR is that real-time PCR: A. can amplify DNA a...
The difference between PCR and real-time PCR is that real-time PCR: A. can amplify DNA a billion-fold within just a few hours, while standard PCR cannot. B. requires primers, while standard PCR does not. C. can determine the DNA sequence, while standard PCR cannot. D. can measure the amount of DNA amplified as the reaction proceeds, while standard PCR cannot. E. uses DNA polymerase, while standard PCR does not.
1. This enzyme replaces RNA primers with a compatible DNA segment. RNA Primase DNA Polymerase III...
1. This enzyme replaces RNA primers with a compatible DNA segment. RNA Primase DNA Polymerase III Topoisomerase DNA Polymerase I 2. This class of enzymes prevent DNA molecules from damaging themselves by relieving strain, untangling, cutting and rejoining strands. They will ride ahead of the replication fork preventing over-winding. Helicase DNA Ligase Topoisomerase RNA polymerase 3. This type of molecule prevents the two separated strands of DNA in a replication bubble from re-establishing their hydrogen bonds ("reannealing"). DNA Polymerase III...
During PCR, the step at which the reaction is held at the optimal temperature for the...
During PCR, the step at which the reaction is held at the optimal temperature for the activity of the DNA polymerase is called the __________________________________ step.
As a reminder, PCR is the technique used to replicate DNA in the lab. PCR is...
As a reminder, PCR is the technique used to replicate DNA in the lab. PCR is based on the way DNA is replicated in cells. During PCR template DNA plus other ingredients are placed in a test tube and the solution goes through repeated cycles of heating and cooling. You can find more information about PCR here. The 'ingredients' of a basic PCR reaction are: DNA template, primer, nucleotides, DNA polymerase, and any salts required for polymerase function (buffer). No...
PCR Questions What gives DNA strands its polarity? To answer, tell me what the “ 5’...
PCR Questions What gives DNA strands its polarity? To answer, tell me what the “ 5’ ” and “ 3’ ” ends are referring to in chemical terms.   Diagram a “template” and the primers for a PCR reaction, including the 5’ and 3’ notations as appropriate. Will the “forward” primer have the same sequence as the coding strand or complement strand? Will the “reverse” primer have the same sequence as the coding strand or complement strand? After running PCR, a...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT