Question

DNA Cloning.
How does having the gene smaller than the vector improve cloning?

Answer 1

A cloning vector is a small piece of DNA, which is taken from a virus, or bacterium or the cell of higher organism that can be maintained stably in another organism, into which a foreign DNA of appropriate size can be inserted for cloning purposes. The vector should contain the features that allow the insertion or removal of foreign DNA.

Cloning vectors are usually selected on the basis of their capacity for the size of the insert DNA. Optimal cloning results are achieved only when the cloned fragment is half the size of the vector. if it is more than half of the size of the vector, after getting inserted into the vector, the vector DNA can not be properly replicated.

if the cloned fragment is smaller than half of the size of vector, then after inserion it can be properly replicated.