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You are cloning an insert DNA into a vector plasmid. a. What is the purpose of...

You are cloning an insert DNA into a vector plasmid.

a. What is the purpose of using the same restriction enzyme for digesting the insert DNA and the vector plasmid? Assume that the restriction enzyme produces sticky ends.

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Answer #1

When restriction enzyme acts on a DNA strand it produces sticky ends. When same enzyme is used in foreign DNA and vector plasmid , it acts on same complementary strand on both and leaving same complementary sticky end on both foreign DNA and vector plasmid. Due to this both foreign DNA and vector plasmid joins easily.

A restriction enzyme is a enzyme that recognizes a specific sequence in DNA and cuts the DNA only at that specific site leaving behind sticky end.

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