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know the steps on how to clone and express gene using restriction emzymes, ligases ,vector, know...

know the steps on how to clone and express gene using restriction emzymes, ligases ,vector, know the vectors for cloning & expression, pcr.
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Answer #1

Cloning vectors are used to amplify target gene sequences. They exhibit a high copy number.
Ex: pGEMT, pBSKS, and pBR322

Expression vectors are used to express the protein from the cloned nucleic acid sequence.
Ex: pMALC2X and pGEX

See the image for the cloning strategy.

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