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short procedure of how to isolate pumpking pigment in TLC plate

short procedure of how to isolate pumpking pigment in TLC plate

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Answer #1

Pumpkin pigment is extracted from the flower of the plant. The corolla is used for extraction of carotenoid the pigment. the flower part is grinded, and the pigment is extracted with equivolume mixture of hexane and acetone. Dried with MgSO4. Washed with water and brine. the carotenoid is dried.

TLC silica gel plates can be used with solvent mixture of pet ether and acetone (9:1) ratio. we can use any primary beta carotene for reference. Place two spots on TLC plate. run the plate in the solvent mixture. TLC can be developed in UV chamber. Rf values can be calcualted.

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