Question

A student’s sample provide one spot on the TLC plate at an Rf value of 0.9;...

A student’s sample provide one spot on the TLC plate at an Rf value of 0.9; however, there are three compounds present in the student’s sample. How could the TLC analysis be adjusted to show all three spots?

Science   Chemistry   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

If the spot at 0.9 Rf is your product please reduce the polarity of the mobile phase to a lower percent and check the TLC. Always preferably the product to be isolated should be at ) 0.45-0.5 Rf value.

In order to view all the three spots adjust the polarity of the solvent, incase all your spots are above 0.9 Rf reduce the polarity (mobile Phase) sufficiently so that all the spots are at lower Rf's

NB: If you can get back to me with exact polarity and which solvents you used as mobile phase I could help you better. Thank you

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Let’s say you have 3 UV active spots in the crude material and co-spot TLC plate....
Let’s say you have 3 UV active spots in the crude material and co-spot TLC plate. One has the same Rf value as the starting material and the other 2 are very different. What could these 2 non-starting material spots be? Use structures and words. Hint: think about the nucleophilic addition of the hydride.
A TLC is run using 2:1 hexane/ethyl acetate on an alumina plate. There were three spots...
A TLC is run using 2:1 hexane/ethyl acetate on an alumina plate. There were three spots all close to the bottom of the plate with low Rf values. How could the TLC be changed to increase the Rf's of the three spots to get better separation?
A student developed a TLC plate by spotting the sample 1 cm from the bottom and...
A student developed a TLC plate by spotting the sample 1 cm from the bottom and allowed the solvent to move up to a point 1 cm from the top. He developed this TLC in 30% ethyl acetate in hexane and got a single spot with Rf value 0.3. If the distance travelled by the solvent front was 8 cm, calculate the distance travelled by the spot. Also, calculate the length of the TLC plate he used to do this...
1. A students performed a TLC experiment on a mixture containing two compounds. However, he observed...
1. A students performed a TLC experiment on a mixture containing two compounds. However, he observed only one spot after visualizing the TLC plate with U.V. light. Give a possible explanation for the student observing only one spot. 2. TLC plates could be made of different particle size of silica. If one used a smaller particle size of silica in the TLC plate, how would that effect the results of a TLC experiment? Explain your answer.
My Post Lab questions are asking me this about my caffeine experiment. "You perform a TLC...
My Post Lab questions are asking me this about my caffeine experiment. "You perform a TLC experiment on a product that you obtained in the lab. When you put it under the UV lamp, you see only one spot. Upon further analysis, however, you realize that there are actually two compounds in the sample. Give two reasons why this could happen, and then suggest how you could modify the experiment to overcome each one" I do not know why this...
if a gas chromotography sample was a mizture of two compounds and producesd a single peak,...
if a gas chromotography sample was a mizture of two compounds and producesd a single peak, how could you increase the resolution so that the two compounds are separated? similarly, if an unknown sample gives one spot in TLC, how do you know that there is no guarantee that it is pure? how can you improve the analysis?
1. The Marquis CST reagent is applied to an unknown drug and the solution turns green....
1. The Marquis CST reagent is applied to an unknown drug and the solution turns green. Next, Cobalt Thiocyanate reagent is applied and the solution turns reddish-orange. For the same unknown drug, Mecke’s reagent turns the solution a greenish black color. The identity of this unknown drug could possibly be _________________________. 2. An unknown sample compound (labeled H), and standards for two known compounds A and B, are subjected to TLC analysis. The results of the TLC analysis are shown...
Boron is the fifth element in the periodic table so it always has 5 protons. However,...
Boron is the fifth element in the periodic table so it always has 5 protons. However, different isotopes of bron have 3,5,6,7, or 8 neutrons in addition to the 5 protons to make up Boron-8, Boron-10, Boron 11, ect. As a research chemist for the Borax Soap Company you have been asked to use a mass spectrometer to determine the relative abundance of different isotopes of born in a sample. You decide to accelerate a beam of singly-ionized born ions....
A one-sample ?-test (Could be EITHER a z- ort-test: complete by hand) Oakland University performs a...
A one-sample ?-test (Could be EITHER a z- ort-test: complete by hand) Oakland University performs a survey of students and learns the average number of hours spent studying per week across the population of all students is 14 hours a week, with a standard deviation of 3 hours. However, a random sample of current SOC 2020 students (N = 100) finds that the mean number of hours spent studying per week is 14.5. You want to know if SOC 2020...
How much money would you have in a year if you put $1,000 in the bank...
How much money would you have in a year if you put $1,000 in the bank at an annual interest rate of 3 percent? How much would you have if you left all of that money in the bank for another year and annual interest rates increased to 4 percent in the second year? How much would you loan your brother-in-law if he said he could repay you $100 in six months, $200 in a year, and $500 in two...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT