Question

In thin-layer chromatography (TLC), the TLC plate may need to be __________________________ in order to identify the migration of particular chemicals while in high-performance liquid chromatography (HPLC), the movement of chemicals through the column is usually detected by ___________________________ . In thin-layer chromatography (TLC), the parameter describing the migration of chemicals up the TLC plate is called the ______________________ . In high- performance liquid chromatography, the parameter describing the movement of “peaks” of chemicals through the column is called the _________________________ .

Answer 1

In thin-layer chromatography (TLC), the TLC plate may need to be spot development in order to identify the migration of particular chemicals while in high-performance liquid chromatography (HPLC), the movement of chemicals through the column is usually detected by chromatogram . In thin-layer chromatography (TLC), the parameter describing the migration of chemicals up the TLC plate is called the Retention factor Rh . In high- performance liquid chromatography, the parameter describing the movement of “peaks” of chemicals through the column is called the Rentention time Tr.