Question

during the isolation and separation of DNA, what role was playe by each of the substance?

1) comb

2) Agarose gel

3) Buffer

Answer 1

1. The comb looks like it name. In the gel electrophoresis , the comb is put before the hot , melted gel is poured. After the gel solidifies , the comb is taken out . The 'teeth' in the comb leave small holes in the gel that we call wells. The wells provide a place to put the tha particles that are used to isolate DNA .

(2) The gel contains pores that allow the DNA particles to move very slowly towards the oppositely charged side of the chamber.

(3) TE buffer is usually used . The purpose of the buffer to stabilize DNA , while protecting it from degradation.

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