Question

How could the accuracy and precision of the DNA size estimation using standard gel electrophoresis with Agarose gel be improved? Give 3 ways!

P.s. Not too technical perhaps considering it's my first year of university question

What I mean is just 3 simple ways to make estimating size of DNA theough gel electrophoresis more accurate or more precise than just manually measuring the distance of DNA bands and comparing it to a reference sample DNA

Answer 1

1) Conc of agarose gel plays important role in estimating the size of DNA. If the size of DNA is larger than % of agrose gel should be less or vive verse.so that movement of molecule should be proper.

2) we can used fresh running buffer with proper concentration salt and ions as these factors also affect movement of DNA

3) DNA sample should be free from any other contamination like RNA,protein salts as this will leads to misleading in results as these molecule will change its mobility.

Thanks..