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We cannot observe the DNA with naked eye during run on agarose gel electrophoresis since DNA...

We cannot observe the DNA with naked eye during run on agarose gel electrophoresis since DNA is colorless and very low amount of DNA is used in this technique.

a) Considering the charge of DNA, what is the direction of DNA migration when it is run on agarose gel electrophoresis? (5 pts)

b) How do we observe the DNA on agarose gel electrophoresis if DNA is colorless? (5 pts) c)

If you want to run an oligonucleotide (25 bp) on agarose gel, what would you do for good separation on the gel?

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Answer #1

A. Since DNA is negatively charged we put on the negative pole of the gel. Under the influence of the electric charge, this DNA will move towards the positive charge of the gel.

B. Since DNA is colorless a tracking dye like bromophenol blue is normally used. It runs with DNA and helps in the visualization of fo DNA bands.

C. The separation of DNA depends on the size of pores of the gel. For good separation the gel is made more concentrated so that is has small pores and DNA becomes more separated.

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