Question

You have access to a PCR machine and all the reagents, (primers, Taq polymerase etc). You...

You have access to a PCR machine and all the reagents, (primers, Taq polymerase etc). You do the PCR reaction on your unknown DNA and you sequenced your PCR product.

The sequence you obtain is the following:

AGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGA CTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTT GAAAAAGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCAC CTCATGGTCATGTTATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTC

What is your unknown?    How did you come to that conclusion?

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Answer #1

In a PCR, we use one of the strand of the DNA as the template and use a primer of short length that is complementary to the DNA strand that needs to be amplified. this occurs on both the strands of the DNA,hence one DNA molecule gives us 2 molecules at the end of 1 cycle. If we have the sequence of the PCR product, it is easy to find the original DNA sequence because it is the exact complementary of this sequence.

Hence the sequence of the original DNA sequence-

TCTTTTGTGTGCAGGTTGAGTCAAACGGACAAAATGTCCAAGCGCTGCACGAGCATGCACCGAAACCTCT

GAGGCACCTCCTCCAGAATAGTCTCCGTGCAGTTGTAGAATTTCTACCGTGAACACCGAATCATCTTCAA

CTTTTTCCGCAAAACGGAGTTGAACTTGTCGGGATACACAAGTAGTTTGCAAGCCTACGAGCTTGACGTG

GAGTACCAGTACAATACCAACTCGACCATCGTCTTGAGCTTCCGTAAG.

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