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Define resolution of a microscope, what is the role of immersion oil used at 100X ?...

Define resolution of a microscope, what is the role of immersion oil used at 100X ? In your own word.

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Answer #1

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Resolution: Resolution is the resolving power of a microscope. It is the capacity of magnifying instruments (lense) to distinguish two objects that are close together. It is determined by following formula-

Resolution (r) = λ/NA

Where,

λ =

NA = numerical aperture

The role of immersion oil: Immersion oil is used in 100x because this oil has high refractive index that increases the numerical aperture of the objective lens and allows the lens to receive more of the light transmitted through the specimen.

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