Question

If observed through the 100x oil immersion objective, what is the size of an object that covers 2/3 of the field of view.

Answer #1

Oil Immersion Lens(100x) has a diameter of field of view equal to 0.2 mm or 200 micrometers

The area of the object that covers complete field of view is calculated by formula

Object size=Diameter of field of view/No.of .objects cross the field of view

- During Microscopic Examination, we examine a part of the whole object so No.of.objects across the field of view is determined by placing the object side by side across the diameter how many objects fit into the diameter or approximating its percentage in 100% of whole size of object

here, diameter of field of view=200micrometer,no.objects. across the field of view=1 (I considered it 1 because the size of the object is not given)

object size=200/1=200micrometers

The size that covers 2/3 is 200x2/3=133.33 micrometers

Define resolution of a microscope, what is the role of immersion
oil used at 100X ? In your own word.

You switch from a 1.44NA 60× oil immersion objective to a 0.67NA
20× air immersion objective. What are the acceptance angles for
each (in degrees)? (Assume the index of refraction of the oil is n
= 1.51.) θ1.44NA 60× = ?(degrees) θ0.67NA 20× =?(degrees) Compare
and comment on the values. Which would you use first to locate the
target area on your specimen?

Explain the problem encountered when magnifying an object with
the oil immersion lens and indicate how immersion oil helps solve
the problem.

Which among A-D is false concerning the
immersion oil technique?
A.
It requires the use of the 100X objective lens on the
microscope
B.
It does not stain the cells.
C.
It minimizes the amount of light refraction allowing more light
to enter the lens
D.
It does not require that a coverslip be used.
E.
None of A-D is false; they are all true statements

Name a purpose of immersion oil in light microscopy; What does
it do? How does it work? What objective lenses on your microscope
should it be used with?

If the field size under a 100x magnification is 0.1 mm in
diameter, what would it be under a 40x magnification? Show the
calculation.
How about a field size of 0.12 mm under a 10x magnification,
what would the field size in mm be under a 400x magnification? Show
the calculation.

17.
What is the benefit of having a large objective lens?
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B) It will produce an inverted image.
C) It will produce a larger image.
D) It will produce a real image.
E) It will produce an image a shorter distance from the lens.
18.
What best describes the image formed by the objective lens?
A) Virtual and reduced.
B) Real and enlarged.
C) Virtual and enlarged.
D) Real and reduced.
19.
What...

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b. If the image of this object is viewed with the eyepiece
adjusted for minimum eyestrain (image at the far point of the eye)
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Build a microscope that can double the size of an object. We
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with focal lengths 2,4,8, and 12 meters.
First choose an objective lens focal length, and a distance to
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Then choose an eyepiece lens focal length and a distance to
place the lens from the objective lens.
Draw your microscope design including a ray diagram.
Your diagram should include:
1. Location and properties...

What is the objective of using probability proportional to size
sampling (PPS) to test account balances?

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