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If observed through the 100x oil immersion objective, what is the size of an object that...

If observed through the 100x oil immersion objective, what is the size of an object that covers 2/3 of the field of view.

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Answer #1

Oil Immersion Lens(100x) has a diameter of field of view equal to 0.2 mm or 200 micrometers

The area of the object that covers complete field of view is calculated by formula

Object size=Diameter of field of view/No.of .objects cross the field of view

  • During Microscopic Examination, we examine a part of the whole object  so No.of.objects across the field of view is determined by placing the object side by side across the diameter how many objects fit into the diameter or approximating its percentage in 100% of whole size of object

here, diameter of field of view=200micrometer,no.objects. across the field of view=1 (I considered it 1 because the size of the object is not given)

object size=200/1=200micrometers

The size that covers 2/3 is 200x2/3=133.33 micrometers

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