Question

\

Eukaryotic cells have much more complex regulatory regions of their genes. Which of the following are involved in regulating or starting eukaryotic transcription? (more than one may apply)

Select one or more:

a. -25 TATA box

b. transcription factors

c. RNA Pol II

d. enhancer regions

e. -35 and -10 consensus sequences

Answer 1

All the following are involved in regulating or starting eukaryotic transcription

1. -25 TATA box- Located at 25 to 30 bp upstreame. It is the binding site of subunit of transcription factor TFIID
2. Transcription factors- are DNA binding proteins which influence trancription. TFIIA, TFIIB, TFIID,TFIIE, TFIIF and TFIIH are examples for eukaryotic transcription factors
3. RNA Pol II- is a protein complex involved in transcription of mRNA, snRNA and miRNA in eukaryotes
4. Enhancer regions- are regions/sequnces of DNA that enhances the transcription. transcription factors bind to enhancer sequences
5. -35 and -10 consensus sequences- consensus sequences are also known as canonical sequences. The initiator region and TATA box comes under -35 and -10 consensus sequences