Question

In eukaryotic cells, which of the following processes require histone remodeling by chromatin remodeling complexes? "select all that apply" explain each answer.

1. RNA interference

2. DNA sliding and positioning

3. DNA synthesis/replication

4. Building a centromere for attachment of microtubules

5. Replacing damaged histone subunits

6. tRNA charging

7. RNA transcription

8. Quickly eliminating the existing 'histone code'

Answer 1

RNA interference - for silencing certain portion the region is kept unopened and rest is to be opened which is carried out by the swi proteins

DNA synthesis and replication- the DNA will be closed by the barrier created by the histons and thus for the access of DNA for replicating and synthesis genes requires it to get opened with the help of chromatin remodeling complex

RNA Transcription- transcription always requires the nucleosome that are bound to the DNA to be opened then only transcription is made possible

Quickly eliminating the existing histon code- histons are arranged with two copies of H3 , H4 , dimer of H2A and H2B. The histon codes are made of any kind of modifications to the histon octamers to the N terminal tail at the H3 end like the acetylation, methylation etc. So to remove any these codes the chromatin remodeling is required.