Question

1) Which of the following statements about enhancer elements is not correct?

Enhancer elements bind to transcription factors that do not have any association with coactivators.

Enhancer elements can be found within introns.

Enhancer elements are regulatory sequences that bind transcriptional activators or signal-regulated TFs

2)Which of the following statements about mediators is incorrect?

The mediator can contain repressor subunits that block transcription.

The coactivator complex and mediator can work in tandem.

The mediator complex connects upstream TFs to the PIC (Pre Initiation Complex).

The mediator complex connects downstream TFs to the PIC (Pre Initiation Complex).

The mediator cannot replace the coactivator.

Enhancer elements are regulatory sequences that can be found downstream from the promoter.

Enhancer elements are regulatory sequences that are distantly located from the promoter.

3) Which of the following statements about eukaryote transcription is correct?

RNA polymerase III is found in nucleoli.

RNA polymerase II transcribes the mitochondrial genes into mRNA, r RNA and tRNA.

RNA polymerase III transcribes RNA precursors for ribosomes.

4)Which of the following statements about Electromobility Shift Assays (EMSA) is correct?

Smaller DNA molecules flow faster down the gel towards the positive pole in EMSA.

EMSA gels contain SDS detergent to maintain the shape of the protein.

Large DNA molecules flow faster down the gel towards the positive pole in EMSA.

EMSA gels cannot be used to determine whether a particular protein has bound to a specific DNA sequence.

RNA polymerase I synthesise the RNA precursors of rRNA.

RNA polymerase II transcribes RNA for ribosomes.

Answer 1

Answer is explained.

a) Incorrect,Enhancer elements bind to transcription factors that do not have any association with coactivators.

b)Correct, Enhancer elements can be found within introns.(As we know that they regulates in genes, which are variable i.e upstream or down stream genes, but also in introns)

c) Correct,Enhancer elements are regulatory sequences that bind transcriptional activators or signal-regulated TFs

Enhancer :- It is a regulatory DNA sequences which is bound by specific proteins known as transcription factors and enhance the transcription of an associated gene.

Regulation of transcription is the most common form of gene control and the activity of transcription factors allows genes to be specifically regulated during development and in different types of cells.