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Could eukaryotic ribosomes accurately translate an mRNA from bacteria? Why or Why not?

Could eukaryotic ribosomes accurately translate an mRNA from bacteria? Why or Why not?

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Answer #1

No, eukaryotic ribosomes can not translate bacterial mRNA

Reason-

Bacteria uses formyl methionine as a start codon but eukaryotes uses only methionine and the eukaryotic ribosome can't add firmyl methionine at P site.

Eukaryotic mRNA is capped at 5' end while prokaryotic mRNA. Does not contains the cap at 5' end. This is the cap of the eukaryotic mRNA at which ribosome binds. If cap is not present ribosome can not bind at mRNA.

Bacterial mRNA contain Kozak sequence while eukaryotic mRNA contains Shine-Dalgarno sequence.  Eukaryotic ribosome are specific for Shine-Dalgarno sequence.

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