Denaturation involves the
breaking of many of the weak such as H bonds
within an enzyme, which are responsible for
the highly ordered structure of the
enzyme. Most enzymes lose
their activity once denatured ,
because substrate can no longer bind to the active
site.
Invertases are enzymes that hydrolyze sucrose into Zn2+ and has
relatively little or no effect on the activity. compared with
control.
The best way to measure purified invertase is to measure the
release of glucose from the sucrose. This can be done either by
measuring an increase in reduced sugar, by dinitro salicylic acid
for example, or by measuring glucose specifically using an
enzymatic assay, such as the glucose oxidase assay.