Question

Please outline the main steps of a column chromatographic separation of a medium polar compound A from another medium compound B and a non-polar chemical (C). B is slightly more polar than A.

Answer 1

we know that TLC will work based on the polarity

compound is more polar that will stick to the silica gel more so it will move slowely through the column

less polar compound will move trough the column more quickly

from the question it is clear that

compound C is nonpolar

compound A and B are very close to each other and B is slightly more polar than A

now

pack the column remove the non polar spot C using non polar mixture

then slowly increase the polarity of mobile phase and then pass until compound A finish.

after finishing this increase the polarity again to remove the more polar spot B