a) In column chromatography, does the outcome of a separation depend on the solvent that is used to dissolve the compound mixture before applying it to the stationary phase? Please explain.
b) If you perform two column chromatographic separations in two columns of equal size, one column packed with a silica gel of smaller particle size than the other, would the outcome of the separation be different? Please explain.
a) In column chromatography, does the outcome of a separation depend on the solvent that is used to dissolve the compound mixture before applying it to the stationary phase? Please explain.
Yes, it depends, since it is the specie that interacts with the mobile phase... It also marks the difference between mobile/stationary phase interactions... Having a solvent that does not interact with a specie and another that does interact will definetively change it.
b) If you perform two column chromatographic separations in two columns of equal size, one column packed with a silica gel of smaller particle size than the other, would the outcome of the separation be different? Please explain.
yes it should, since the siliga gel in one column will have a larger area to interact, therefore more contact between species and the stationary phase, which wil change the Rf value
Get Answers For Free
Most questions answered within 1 hours.