Question

1. If you load the mixture of compounds using more than a "minimal amount of CH₂Cl₂", what will happen to your separation?

A. The seperation will be poor since CH2Cl2 is a less polar solvent than petroleum ether.

B. The seperation will be improved since CH2Cl2 is a more polar solvent than petroleum ether

C. The seperation will be improved since CH2Cl2 is a less polar solvent than petroleum ehter

D. The seperation will be poor since CH2Cl2 is a more polar solvent than petroleum ehter

E. The seperation will be poor since CH2Cl2 is a nonpolar solvent

2. Place the following events in correct order:

Loading,evaporation, TLC fraction checking, elution, combining like fractions, packing.

3. The chromotagraphic term for the solvent that partitions the analyte along the column of stationary phase:

a. stationary phase/adsorbent

b. mobile phase/eluent

4. The chromatographic term for the solid material to which the analyte molecules adhere while chromatography is being performed:

a. stationary phase/adsorbent

b. mobile phase/eluent

5. The term used in reference to one of many portions of solvent isolated from the end of a chromatography column:

a. sample

b. fraction

c. spot

Answer 1

1. If you load the mixture of compounds using more than a "minimal amount of CH2Cl2", what will happen to your separation?

Answer= D. The seperation will be poor since CH₂Cl₂ is a more polar solvent than petroleum ether.

2. The following events in correct order are as follows--

Answer = Packing, Loading ,elution, TLC fraction checking, combining like fractions ,evaporation.

3.  The chromotagraphic term for the solvent that partitions the analyte along the column of stationary phase:-

Answer= b. mobile phase/eluent

4. The chromatographic term for the solid material to which the analyte molecules adhere while chromatography is being performed:

Answer= a. stationary phase/adsorbent

5. The term used in reference to one of many portions of solvent isolated from the end of a chromatography column:

Answer= b. fraction