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What causes different substances to move up a TLC slide? What causes different substances to separate...

What causes different substances to move up a TLC slide? What causes different substances to separate as they move up a TLC slide?
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TLC is nothing but thin layer chromatography. This thin layer is of silica (SiO2). Different organic compound has different functional group. NH2, COOH, OMe, SH, NMe, PH, Ethyl, COOEt, CONH2 etc. Since you studied Hydrogen bonding. This functional group of the substances forms hydrogen bonding with the silica. When you run your TLC plats due to formation and breaking of new bond THE substance run on the TLC plate. Since polar functions like NH2. COOH are strongly bind with silica hence run slowly and those which are weakly bound with silica like aliphatic moiety, ether linkages, OMe functions are run faster . Hence different substances get separated on the TLC due to their functional groups means hydrogen bonding with the silica.

Solvent rises on TLC plate due to the capillary effect.

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