Question

Why is it possible to detect nicotinic acid with UV spectrometry instead of using a fluorimeter...

Why is it possible to detect nicotinic acid with UV spectrometry instead of using a fluorimeter or atomic absorption spectrometer? Support your answer.

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Answer #1

Fluorimetry is the quantitative study of fluorescence of fluorescent molecules, while Atomic absorption spectrometry is the analytical technique using which we can measure the concentration of elements. However UV spectrometry is the technique which can used to detect functional groups in an organic molecule, quantitative and qualitative analysis, detection of conjugation in a compound etc.. Hence Nicotinic acid can be best detected by this technique by giving its characteristic peak at a particular wavelength.

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