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Compare and contrast northern blot with in situ hybridization.

Compare and contrast northern blot with in situ hybridization.
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The northern, blot or RNA blotis a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample.

*Only mRNA from cell types that are synthesizing protein will hybridize to the probe.

*It is also useful in detection of mRNA transcript size.

* Blots can be stored several years and reprobed if necessary.

Insitu_hybridization :is a type of hybridization that uses a labeled complementary DNA, RNA or modified nucleic acids strand (i.e., probe) to localise a specific DNA or RNA sequence in a portion.

* it is the used reveal the location of specific nucleic acid sequences on chromosomes or in tissues.

* It include single cell analysis.

* It can be used when no antibody when no antibody exist for a protein.

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