Question

What is an in-situ hybridization? What can it tell you about the function of the ABC genes?

Answer 1

In-situ hybridization is used to reveal location of nucleic acids sequences on chromosomes or in tissue sections and cells, it involves hybridization of a labeled RNA or DNA probe, to a known target mRNA or DNA sequence within fixed tissue sections or in cells. The radiolabelled or fluorescently labeled probe is then localized and quantified using autoradiography or fluoroscence microscopy.

ABC genes act alone and in various different combinations to specify each of the four whorls of a flower.