Question

What are some limitations to a serial dilution lab? As well as possible errors and how they can be avoided?

Answer 1

Answer :

Many laboratory protocols requires serial dilution of reagents or compounds.

Serial dilution faces major challenges.

Error propagation across columns or rows. They are procedural errors, by inaccurate transfer, dilution and inadequate mixing.

Result is highest dilutions will have maximum error.

This error can be compensated by longer mixing times are required. This increases time required to do serial dilution procedure.

With raising of mix tip height precision increased.

Mix liquid class setting modification with mixing velocity increased accuracy.

Dynamic tip extension and retraction also improved precision. Tips dipped more depth and retracted during dispensing.