five-fold serial dilution was made, so dilution factor at each step=5
dilution factor in the third tube ( third step)= dilution factor in tube 1 dilution factor in tube 2 dilution factor in tube 3=555=125
so the concentration in the third tube= original concentration/dilution factor in the third tube
= 2 g/ mL/125
= 0.016 g/mL
concentration of the third tube = 0.016 g/mL
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