Question

Serial dilutions Please draw how to prepare this serial dilution

prepare a series of p nitrophenyl phosphate concentrations from 1x10^2 M from to 3.125x10^-4 using the DEA buffer as diluent. do this by srial diluting the 1x10^-2M stock by 1/2 so you end up with 2 ml each of p-nitrophenyl phosphate concentrations of 5x10^-3M, 2.5X10^-3M, 1.25x10^-3M, 6.25x10^-4M and 3.125x10^-4M

Answer 1

The diluton scheme starts by diluting at 1/2 each serial dilution starting from the original solution of [p-nitrophenyl-phosphate]_0​ of 1.0.10^-2​M. The first dilution number 0 is with 4 ml of 1.0.10^-2​M. Next dilutions starts with 2.0ml from each previous dilution and adding 2 ml of DEA in each succesive dilution.​​​

The scheme is:

In dilution 0: [p nitrophenylphosphate]_0​= 1.0.10^-2​M​​. It must not need DEA buffer

​It needs 2.0ml of DEA

It also needs 2.0 ml of DEA

This dilution scheme and calculus continue until dilution number 5