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what would it mean if there was growth in the LB/amp +plasmid plate only and if...

what would it mean if there was growth in the LB/amp +plasmid plate only and if the bacteria glowed?

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Answer #1

This plasmid contains an ampicillin-resistance gene in addition to the GFP gene. Ampicillin is an antibiotic and works by preventing E.coli from constructing cell walls, thereby killing the bacteria. When the ampicillin-resistance gene is present, it directs the production of an enzyme that blocks the action of the ampicillin, and the bacteria are able to survive. Bacteria without the plasmid and, hence, the resistance gene are unable to grow on a plate containing ampicillin in the medium, and only the transformants will survive.

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