Question

Transformation of E. coli with the plasmid (pGLO)

1. In LB agar only plate

	Control tube Without plasmid (-)	Experimental tube with plasmid (+)
Will you get growth?         (yes or no)
If growth, will transformants be present?     (yes or no)
Will the colony fluoresce under UV light?         (Yes or no)

2. In LB agar with ampicillin and arabinose

	Control tube Without plasmid (-)	Experimental tube with plasmid (+)
Will you get growth?         (yes or no)
If growth, will it contain transformants?     (yes or no)
Will the colony fluoresce under fluorescent light? (Yes, no or NA)

Answer 1

Plasmids which contains both the GFP gene and AMP gene (pGLO) . The plasmid transferred cells will be grow in culture media which contains ampicillin. For the bacteria which has to fluorescence under the UV light arabinose should be added in the media which helps to turn on the gene for the production of glowing fluorescent protein. And it can grow in the presence of antibiotic ampicillin because the plasmid has amp resistance gene.

1. In LB agar only plate

Control tube without plasmid

1. yes

2. No

3. No

Experimental tube with plasmid

1. Yes

2. Yes

3. No

2. LB agar with ampicillin and arabinose

Control tube without plasmid

1. No

2. NO

3. No

Experimental tube with plasmid

1. Yes

2. Yes

3. Yes,as GFP gene is present in the plasmid it wi fluoresce in fluorescent light