Question

Predict what you expect to see each of the two NA AMP plates shown in the...

Predict what you expect to see each of the two NA AMP plates shown in the Transformation Protocol in both visible light and UV light. You should have 4 predictions. We have provided a few examples of answers to help guide you.

E. coli + pGRN plasmid on nutrient agar (NA)

E. coli + pGRN plasmid on nutrient agar with ampicillin (AMP)

HINT: For the 3rd data row (+pGRN AMP, visible), you should predict the number of colonies relative to the 1000 predicted first data row. So - do you expect more than 1000, less than 1000 or equal to 1000.

pGRN added?

Plate

Light

Approximate number of colonies and color

+

NA

visible

Example: 1000 colonies, all white

+

NA

UV

Example: 1000 colonies, only a few glow green

+

AMP

visible

+

AMP

UV

Give an explanation for your two predictions – both numbers and color.

Homework Answers

Answer #1
pGRN added?

Plate

Light

Approximate number of colonies and color

+

NA

visible

Example: 1000 colonies, all white

+

NA

UV

Example: 1000 colonies, only a few glow green

+

AMP

visible

The colonies are must less than 1000 (transformation rate is very low so that number may be double digit),

COLOUR: WHITE COLOUR

+

AMP

UV

Less than 1000 - colour green.

pGRN plasmid has ampicillin resistance gene, the succ successful transformed bacteria can survive in the medium with AMPICILLIN. But the number of transformed cells are very low in number

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Predict what you expect to see each of the two NA AMP plates shown in the...
Predict what you expect to see each of the two NA AMP plates shown in the Transformation Protocol in both visible light and UV light. You should have 4 predictions. We have provided a few examples of answers to help guide you. E. coli + pGRN plasmid on nutrient agar (NA) E. coli + pGRN plasmid on nutrient agar with ampicillin (AMP) HINT: For the 3rd data row (+pGRN AMP, visible), you should predict the number of colonies relative to...
When biologists conduct transformation experiments, they are concerned that the cells receiving the plasmid could already...
When biologists conduct transformation experiments, they are concerned that the cells receiving the plasmid could already be resistant to the antibiotic used. For example, in this experiment, it is possible that a small fraction of the E. coli cells were already resistant to ampicillin. If true, the colonies seen on the NA-AMP plates in part A would NOT be actual transformants, but rather – just natural variants of the E. coli cells. Due to this concern, experimental controls are performed:...
Another researcher performed the same experiment and obtained the results shown in the table below. pGRN...
Another researcher performed the same experiment and obtained the results shown in the table below. pGRN added? Plate Light number of colonies + NA visible 10,000 + NA UV 10,000 + AMP visible 50 + AMP UV 50 - NA visible 10,000 - NA UV 10,000 - AMP visible 50 - AMP UV 50 Explain these results, focusing on the shaded rows.Do you think this researcher’s transformation was successful?Explain your answer.
As a general principle, in order to validate results in scientific experiments, scientists use controls. A...
As a general principle, in order to validate results in scientific experiments, scientists use controls. A control is a group that is designed to demonstrate that the results/changes observed in an experiment were because of the procedure you performed. It sets a baseline value with which the scientist can compare with experimental sample and interpret results, as was demonstrated in the previous question. In this question, we are asking you to describe the control experiments, using the same transformation protocol,...
BACTERIAL TRANSFORMATION PROTOCOL AND QUESTION 1. You have a tube of pGLO plasmid at a concentration...
BACTERIAL TRANSFORMATION PROTOCOL AND QUESTION 1. You have a tube of pGLO plasmid at a concentration of 15 ng/uL 2. Label two microfuge tubes; one with "+ pGLO" and the other "-pGLO" Add 250ul of cold transformation solution to each tube and place the tubes on ice. 3. Transfer 10 ul of the pGLO plasmid DNA only to the "+ pGLO" tube containing transformation solution and cells. Mix the DNA solution with the cell suspension in the tube. 4. Add...
You have a tube of pGLO plasmid at a concentration of 15 ng/uL and you perform...
You have a tube of pGLO plasmid at a concentration of 15 ng/uL and you perform transformation experiments using protocol below. The protocols use E. coli that are made competent (i.e. – able to take up exogenous DNA).   Protocol is outlined below: 1 uL of pGLO was added to 100 uL of competent cells in transformation buffer (these cells had been made competent by a different procedure than the one we used in class but we don’t need to concern...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT