Question

Predict what you expect to see each of the two NA AMP plates shown in the Transformation Protocol in both visible light and UV light. You should have 4 predictions. We have provided a few examples of answers to help guide you.

E. coli + pGRN plasmid on nutrient agar (NA)

E. coli + pGRN plasmid on nutrient agar with ampicillin (AMP)

HINT: For the 3^rd data row (+pGRN AMP, visible), you should predict the number of colonies relative to the 1000 predicted first data row. So - do you expect more than 1000, less than 1000 or equal to 1000.

pGRN added?	Plate	Light	Approximate number of colonies and color
+	NA	visible	Example: 1000 colonies, all white
+	NA	UV	Example: 1000 colonies, only a few glow green
+	AMP	visible
+	AMP	UV

Give an explanation for your two predictions – both numbers and color.

Answer 1

pGRN added?	Plate	Light	Approximate number of colonies and color
+	NA	visible	Example: 1000 colonies, all white
+	NA	UV	Example: 1000 colonies, only a few glow green
+	AMP	visible	The colonies are must less than 1000 (transformation rate is very low so that number may be double digit), COLOUR: WHITE COLOUR
+	AMP	UV	Less than 1000 - colour green.

pGRN plasmid has ampicillin resistance gene, the succ successful transformed bacteria can survive in the medium with AMPICILLIN. But the number of transformed cells are very low in number